Characterization of a brain-specific nuclear LIM domain protein (FHL1B) which is an alternatively spliced variant of FHL1
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摘要We have amplified and sequenced a novel, alternatively spliced variant of a human gene coding for the four-and-a-half LIM domain protein 1 (FHL1). This gene is located at chromosome Xq27 and the spliced variant is named FHL1B. The ORF of FHL1B cDNA codes for a LIM-only protein that possesses a zinc finger and three tandem repeats of LIM domains at the N-terminus with an active bipartite nuclear localization signal (NLS) motif and a possible RBP-J binding region at the C-terminus. FHL1B and FHL1 have the same N-terminal three-and-a-half LIM domains but different C-terminal protein sequences, due to the presence of an additional alternative exon 4b in FHL1B causing a frame-shift in the 3'coding region. RT-PCR results revealed that the expression of FHL1 is not restricted in skeletal muscle and heart, but is widely distributed in other tissues, including brain, placenta, lung, liver, kidney and pancreas, albeit as a low abundance transcript. In contrast, FHL1B is specifically expressed in brain. The C-terminal alternative region in FHL1B is sufficient to localize FHL1B in the nucleus of mammalian cell. FHL1B is probably related to neural differentiation and certain fragile X syndrome. (C) 1999 Elsevier Science B.V. All rights reserved.
著者Lee SMY, Li HY, Ng EKO, Or SMW, Chan KK, Kotaka M, Chim SSC, Tsui SKW, Waye MMY, Fung KP, Lee CY
期刊名稱Gene
出版年份1999
月份9
日期3
卷號237
期次1
出版社ELSEVIER SCIENCE BV
頁次253 - 263
國際標準期刊號0378-1119
電子國際標準期刊號1879-0038
語言英式英語
關鍵詞brain; brain cDNA; FHL1B; RBP-J; SLIM1; Xq27; zinc finger protein
Web of Science 學科類別Genetics & Heredity; GENETICS & HEREDITY

上次更新時間 2020-02-08 於 04:10