Isolation of a trypsin inhibitor with deletion of N-terminal pentapeptide from the seeds of Momordica cochinchinensis, the Chinese drug mubiezhi
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摘要A trypsin inhibitor, MCCTI-1, with a molecular weight of 3479 Da as determined by mass spectrometry, was isolated from Momordica cochinchinensis seeds with a procedure involving extraction with 5% acetic acid, ammonium sulfate precipitation, ion exchange chromatography on CM-Sepharose and reverse-phase high performance liquid chromatography. The sequence of its first 13 N-terminal amino acid residues was ILKKCRRDSDCPG which was about 85% identical with the sequence of trypsin inhibitor MCTI-1 from Momordica a charantia Linn, When compared with the sequences of most other squash family trypsin inhibitors, the sequence of MCCTI-1 was characterized by the deletion of a pentapeptide from the N-terminus. Trypsin inhibitors also existed in seeds of some hitherto uninvestigated Cucurbitaceae species. (C) 1999 Elsevier Science Ltd. All rights reserved.
著者Huang B, Ng TB, Fong WP, Wan CC, Yeung HW
期刊名稱International Journal of Biochemistry and Cell Biology
出版年份1999
月份6
日期1
卷號31
期次6
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次707 - 715
國際標準期刊號1357-2725
電子國際標準期刊號1878-5875
語言英式英語
關鍵詞amino acid sequence; Cucurbitaceae; Momordica cochinchinensis; squash family trypsin inhibitors
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

上次更新時間 2020-26-11 於 01:53