Inhibition of glucose transporter gene expression by antisense nucleic acids in HL-60 leukemia cells
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AbstractGlucose is the basic source of energy for mammalian cells. The energy-independent transport of glucose down its concentration gradient is mediated by the facilitative glucose transporter family (GLUT). It has long been recognised that glucose transporter genes are overexpressed in many human cancer cells, to help provide extra energy for the rapid growth of cancer cells. In the present study, antisense oligonucleotides and plasmid-derived antisense RNA against GLUT-1 gene were synthesized and transfected into human leukemia HL-60 cells to investigate the effect of these antisense nucleic acids on tumour growth. Our results show that antisense nucleic acids inhibited the proliferation of HL-60 cells by 50-60% and the mRNA expression of GLUT-1 gene was suppressed as detected by Northern hybridization.
All Author(s) ListChan JYW, Kong SK, Choy YM, Lee CY, Fung KP
Journal nameLife Sciences
Year1999
Month5
Day28
Volume Number65
Issue Number1
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Pages63 - 70
ISSN0024-3205
eISSN1879-0631
LanguagesEnglish-United Kingdom
Keywordsantisense nucleic acid; glucose transporter; HL-60 cells
Web of Science Subject CategoriesMedicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

Last updated on 2020-02-07 at 04:49