Activity of voltage-gated K+ channels is associated with cell proliferaton and Ca2+ influx in carcinoma cells of colon cancer
Publication in refereed journal

香港中文大學研究人員

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摘要Cell proliferation of carcinoma cells DLD-1 derived from colon cancer as measured by [H-3] thymidine incorporation was drastically reduced in the presence of 4-aminopyridine, an inhibitors of voltage-gated K+ channel. A number of nonspecific K+ channel inhibitors including TPeA, TEA, verapamil and diltiazem also inhibited [H-3] incorporation at the concentration reported to inhibit voltage-gated K+ channels. The presence of voltage-gated K+ channels was confirmed by reverse transcription-PCR and cDNA sequencing. Charybdotoxin and iberiotoxin, inhibitors for Ca2+-sensitive K+ channel, and glibenclamide, a specific inhibitor for ATP-sensitive K+ channel, did not have effect on cell proliferation. These experiments suggested a critical role of voltage-gated K+ channels in proliferation of colon cancer cells. Mechanism of action of K+ channel activity in cell proliferation was explored by studying the relationship between the K+ channel activity and Ca2+ entry. The results from experiments indicated that K+ channel inhibitors blocked [Ca2+](i) influx. Therefore, it is likely that K+ channel activity may modulate Ca2+ influx into colon cancer cells, and subsequently modulate the proliferation of these cells.
著者Yao XQ, Kwan HY
期刊名稱Life Sciences
出版年份1999
月份5
日期28
卷號65
期次1
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次55 - 62
國際標準期刊號0024-3205
電子國際標準期刊號1879-0631
語言英式英語
關鍵詞Ca2+ influx; cell proliferation; colon cancer; K-v channels
Web of Science 學科類別Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

上次更新時間 2020-14-09 於 03:13