Studies on purification of a lectin from fruiting bodies of the edible shiitake mushroom Lentinus edodes
Publication in refereed journal

香港中文大學研究人員

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摘要A lectin, with a specificity for N-acetylgalactosamine and N-acetylglucosamine and a molecular weight of 43 kDa, was isolated from fruiting bodies of the edible shiitake mushroom Lentinus edodes. The purification procedure entailed extraction with aqueous buffer, ammonium sulfate precipitation, gel filtration on Sephadex G-100 and affinity chromatography on N-acetylgalactosamine-agarose. The lectin was unique in that it was tenaciously bound on anion exchangers including DEAE-cellulose, DEAE-Sepharose, DEAE-Sephadex, Q-Sepharose, Dowex and PEI-cellulose and also on hydroxyapatite and phenyl Sepharose. It was largely unadsorbed on cation exchangers including CM-cellulose, CM-Sepharose, SP-Sepharose and Amberlite. and also on protein G-Sepharose, Red Sepharose and Affi-gel Blue gel, wheat germ lectin-Sepharose and p-aminophenyl-D-glucopyranoside agarose. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.
著者Wang HX, Ng TB, Ooi VEC
期刊名稱International Journal of Biochemistry and Cell Biology
出版年份1999
月份5
日期1
卷號31
期次5
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次595 - 599
國際標準期刊號1357-2725
電子國際標準期刊號1878-5875
語言英式英語
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

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