Involvement of endothelium/nitric oxide in vasorelaxation induced by purified green tea (-)epicatechin
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摘要The present study investigated the involvement of endothelial nitric oxide in relaxation induced by purified green tea (-)epicatechin in rat isolated mesenteric arteries. (-)Epicatechin caused both endothelium-dependent and -independent relaxation. N-G-Nitro-L-arginine methylene (L-NAME, 10 mu M) and methylene blue (10 mu M) significantly attenuated (-)epicatechin-induced relaxation in endothelium-intact tissues. L-Arginine (1 mM) partially antagonized the effect of L-NAME. (-)Epicatechin-induced relaxation was inhibited by Rp-guanosine 3',5'-cyclic monophosphothioate triethylamine. In contrast, indomethacin and glibenclamide had no effect. (-)Epicatechin (100 mu M) significantly increased the tissue content of cyclic GMP and N-G-nitro-L-arginine (100 mu M) or removal of the endothelium abolished this increase. (-)Epicatechin (100 mu M) induced an increase in intracellular Ca2+ levels in cultured human umbilical vein endothelial cells. Iberiotoxin at 100 nM attenuated (-)epicatechin-induced relaxation in endothelium-intact arteries and this effect was absent in the presence of 100 mu M r-NAME. In summary, (-)epicatechin-induced endothelium-dependent relaxation is primarily mediated by nitric oxide and partially through nitric oxide-dependent activation of iberiotoxin-sensitive K+ channels. In addition, there may be a causal link between increased Ca2+ levels and nitric oxide release in response to (-)epicatechin. (C) 1999 Elsevier Science B.V. All rights reserved.
著者Huang Y, Chan NWK, Lau CW, Yao XQ, Chan FL, Chen ZY
期刊名稱BBA - General Subjects
出版年份1999
月份4
日期19
卷號1427
期次2
出版社ELSEVIER SCIENCE BV
頁次322 - 328
國際標準期刊號0304-4165
電子國際標準期刊號1872-8006
語言英式英語
關鍵詞(-)epicatechin; artery; endothelium; green tea; nitric oxide; relaxation
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

上次更新時間 2020-26-11 於 01:50