Localization and distribution of pituitary adenylate cyclase-activating polypeptide in the rat epididymis
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摘要Previous investigation has provided evidence for the control of electrogenic chloride secretion by pituitary adenylate cyclase-activating polypeptide (PACAP) across the rat epididymal epithelium using electrophysiological measurement of transepithelial transport in cultured epididymal system. Hence, it suggests that epididymal and sperm functions are subject to control by a local PACAP system in the rat epididymis. In the present study, localization and distribution of PACAP in the rat epididymal duct was studied by an indirect immunofluorescence technique in conjunction with confocal laser scanning microscopy. Immunoreactivity for PACAP was found in all regions of the epididymal duct. However, the intensity of immunoreactivity for PACAP was stronger in the caput and corpus regions when compared to that of the cauda epididymidis. Much weaker immunostaining for PACAP, as compared to those found in other regions, was observed in the cauda epididymidal tubules which are in close proximity to the vas deferens. No immunoreactivity for PACAP was found in epididymal spermatozoa. Together with the previous finding, the present results suggest that PACAP may exhibit a regional difference in its expression along the epididymal duct and it may act in a paracrine or autocrine fashion in the regulation of epididymal chloride secretion and hence fluid secretion, thus regulating epididymal and sperm functions along the epididymal duct. (C) 1998 Academic Press.
著者Leung PS, Wong TP, Wong PYD, Chan HC
期刊名稱Cell Biology International
出版年份1998
月份1
日期1
卷號22
期次3
出版社ACADEMIC PRESS LTD
頁次193 - 198
國際標準期刊號1065-6995
電子國際標準期刊號1095-8355
語言英式英語
關鍵詞epididymis; immunocytochemistry; PACAP; sperm
Web of Science 學科類別Cell Biology; CELL BIOLOGY

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