Stimulation of HCO3- secretion across cystic fibrosis pancreatic duct cells by extracellular ATP
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香港中文大學研究人員

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摘要Previous studies have demonstrated that extracellular ATP is able to activate Ca2+-dependent Cl- channels which may be important in circumventing the defective cAMP-dependent pancreatic ductal HCO3- secretion in cystic fibrosis (CF), The present study further investigated the effect of extracellular ATP on the stimulation of HCO3- secretion across CF pancreatic duct cells (CFPAC-1), Cells were grown in culture plate inserts which enabled the access of a pH microelectrode to the apical compartment. Changes in apical pH upon stimulation by extracellular ATP, as an indication of HCO3- secretion, were measured. ATP induced a rapid increase in apical pH, which reached a plateau with an averaged pH unit of 0.3 higher than that measured in unstimulated cells. The effect of ATP was concentration dependent. The ATP-induced change in pH could be blocked by apical addition of Cl- channel blockers, indicating that activation of apical Cl- channel is vital for HCO3- secretion by the pancreatic duct cells. Together with the previous finding, the present study suggests that HCO3- secretion may be stimulated in CF pancreatic duct cells by extracellular ATP via a cAMP-independent pathway.
著者Cheung CY, Wang XF, Chan HC
期刊名稱BIOLOGICAL SIGNALS AND RECEPTORS
出版年份1998
月份11
日期1
卷號7
期次6
出版社KARGER
頁次321 - 327
國際標準期刊號1422-4933
語言英式英語
關鍵詞ATP; CFPAC-1; cystic fibrosis; HCO3-; pancreatic duct; pH; secretion
Web of Science 學科類別Biochemistry & Molecular Biology; Biophysics; Cell Biology

上次更新時間 2020-24-10 於 03:34