Ca2+ is released from the nuclear tubular structure into nucleoplasm in C6 glioma cells after stimulation with phorbol ester
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摘要It is sell established that cellular Ca2+ is an important messenger that controls many nuclear functions but the source of nuclear Ca2+ is far from clear. It has long been thought that Ca2+ is translocated from the cytosol over a long distance to activate the nuclear transcription machinery, Ho,ever, this model is at best an incomplete one. With the aid of confocal microscopy, we observed tubules extended deep inside the nucleus of C6 cells in agreement with previous studies (Fricker et al, (1997) J, Cell Biol, 136, 531-544), When cells were stimulated with phorbol 12-myristate 13-acetate or phorbol 12,13-diacetate, Ca2+ was released from these tubules, DiOC6(3), a vital marker for intracellular membranes, stained the tubule in the nucleus of the same cell used for Ca2+ imaging, Moreover, results from labelling the cells with rhodamine 123 further indicate that the tubule was formed by a double-membraned imagination with mitochondria inside. Studies with acridine orange showed that chromatin was excluded from the tubules, Taken together, our results demonstrate that the nuclear tubule is a structural entity responsible for the release of Ca2+ into the nucleoplasm after stimulation with phorbol ester, (C) 1998 Federation of European Biochemical Societies.
著者Lui PPY, Lee CY, Tsang D, Kong SK
期刊名稱FEBS Letters
出版年份1998
月份7
日期31
卷號432
期次1-2
出版社ELSEVIER SCIENCE BV
頁次82 - 87
國際標準期刊號0014-5793
電子國際標準期刊號1873-3468
語言英式英語
關鍵詞confocal microscopy; invagination; nuclear Ca2+; nuclear tubular structure
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY

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