Development of an enzyme-linked immunosorbent assay with monoclonal antibody for quantification of homovanillic in human urine samples
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香港中文大學研究人員

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摘要A monoclonal antibody to homovanillic acid (HVA) was prepared by synthesis of a HVA-protein conjugate (HVA-ovalbumin) as an immunogen, immunization of mice, and the subsequent hybridization technique. Monoclonal antibodies were screened on the basis of sensitivity, specificity, and accuracy. An indirect ELISA was developed for quantification of HVA in human urine. The assay was characterized and shown to have high specificity, with cross-reactivities to vanillylmandelic acid and normetanephrine at 0.18% and <0.1%, respectively. The assay coefficients of variation were <10% within the working range of 0.5-40 mg/L. Initial results from testing urine samples of patients with neuroblastoma and other diseases were validated by HPLC, suggesting that this ELISA method is a reliable and convenient system for quantification of HVA in urine and can be used in the mass screening of neuroblastoma in infants.
著者Shi RZ, Ho YP, Yeung JHK, Or PMY, To KKW, Lau MWM, Arumanayagam M
期刊名稱Clinical Chemistry
出版年份1998
月份8
日期1
卷號44
期次8
出版社AMER ASSOC CLINICAL CHEMISTRY
頁次1674 - 1679
國際標準期刊號0009-9147
電子國際標準期刊號1530-8561
語言英式英語
Web of Science 學科類別Medical Laboratory Technology; MEDICAL LABORATORY TECHNOLOGY

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