Inhibition of glycohydrolase enzymes by aqueous extracts of Chinese medicinal herbs in a microplate format
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摘要A microplate assay, for use with a variety of glycohydrolase enzymes, was developed to aid the screening of Chinese medicinal herb extracts for the presence of potential anti-viral and anti-lymphoma compounds. The microplate assay method described offers greater convenience, speed and reproducibility over existing methods. The enzymes tested were alpha-glucosidase, beta-glucosidase and beta-glucuronidase. The assay can be easily adapted for use with other glycohydrolase enzymes. Of the 12 herb extracts examined four did not inhibit any of the enzymes (<50% inhibition), one inhibited alpha-glucosidase only (>50% inhibition), six inhibited beta-glucuronidase only, and one inhibited both alpha-glucosidase and beta-glucuronidase. None of the extracts were capable of inhibiting beta-glucosidase to any significant extent.
著者Collins RA, Ng TB, Fong WP, Wan CC, Yeung HW
期刊名稱BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
出版年份1997
月份9
日期1
卷號42
期次6
出版社ACADEMIC PRESS AUST
頁次1163 - 1169
國際標準期刊號1039-9712
語言英式英語
關鍵詞Chinese herbs; enzyme assay; glycohydrolase; inhibition; microplate
Web of Science 學科類別Biochemistry & Molecular Biology

上次更新時間 2020-14-10 於 00:54