Inhibition of glycohydrolase enzymes by aqueous extracts of Chinese medicinal herbs in a microplate format
Publication in refereed journal


Full Text

Times Cited
Web of Science57WOS source URL (as at 23/10/2020) Click here for the latest count

Other information
AbstractA microplate assay, for use with a variety of glycohydrolase enzymes, was developed to aid the screening of Chinese medicinal herb extracts for the presence of potential anti-viral and anti-lymphoma compounds. The microplate assay method described offers greater convenience, speed and reproducibility over existing methods. The enzymes tested were alpha-glucosidase, beta-glucosidase and beta-glucuronidase. The assay can be easily adapted for use with other glycohydrolase enzymes. Of the 12 herb extracts examined four did not inhibit any of the enzymes (<50% inhibition), one inhibited alpha-glucosidase only (>50% inhibition), six inhibited beta-glucuronidase only, and one inhibited both alpha-glucosidase and beta-glucuronidase. None of the extracts were capable of inhibiting beta-glucosidase to any significant extent.
All Author(s) ListCollins RA, Ng TB, Fong WP, Wan CC, Yeung HW
Journal nameBIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
Year1997
Month9
Day1
Volume Number42
Issue Number6
PublisherACADEMIC PRESS AUST
Pages1163 - 1169
ISSN1039-9712
LanguagesEnglish-United Kingdom
KeywordsChinese herbs; enzyme assay; glycohydrolase; inhibition; microplate
Web of Science Subject CategoriesBiochemistry & Molecular Biology

Last updated on 2020-24-10 at 03:15