Position 120-123, a potential active site of trichosanthin
Publication in refereed journal

香港中文大學研究人員

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摘要Trichosanthin (TCS) is a type-I ribosome-inactivating protein (RIP) with wide spectrum of biological and pharmacological activities. In the present study, a potential site on the TCS molecule (position 120-123) is identified which may be important for the biological activities of TCS. By using site-directed mutagenesis, position 120-123 of TCS was either deleted or changed from Lys-Ile-Arg-Glu (hydrophilic) to Ser-Ala-Gly-Gly (hydrophobic). Deletion of these residues rendered a TCS molecule completely deprived of ribosome inactivating activity, while hydrophobic replacement caused 4000-fold decrease in ribosome inactivating activity. The abortifacient activity of these two mutants was retained with decreased potency. This implies that position 120-123 of the native TCS molecule plays a critical role in maintaining its biological activity.
著者Nie HL, Cai XF, He XH, Xu LH, Ke XY, Ke YB, Tam SC
期刊名稱Life Sciences
出版年份1998
月份1
日期2
卷號62
期次6
出版社PERGAMON-ELSEVIER SCIENCE LTD
頁次491 - 500
國際標準期刊號0024-3205
電子國際標準期刊號1879-0631
語言英式英語
關鍵詞active site; ribosome-inactivating protein; site-directed mutagenesis; trichosanthin
Web of Science 學科類別Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

上次更新時間 2020-22-10 於 01:58