Immunomodulatory Activity of Polysaccharide-Protein Complex from the Mushroom Sclerotia of Polyporus rhinocerus in Murine Macrophages
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要A novel water-soluble polysaccharide–protein complex (PRW1) isolated from the sclerotia of an edible mushroom Polyporus rhinocerus which was purified by membrane ultrafiltration could significantly activate murine macrophages RAW264.7 in vitro. PRW1 had a molecular weight of less than 50 kDa and was found to be a highly branched heteropolysaccharide–protein complex composed of 45.7 ± 0.97% polysaccharide and 44.2 ± 0.41% protein. Based on the results of total acid hydrolysis, methylation analysis, and Fourier transform infrared spectroscopy, the carbohydrate moiety of PRW1 was found to be a β-d-mannoglucan with its backbone containing →1)-d-Glcp-(4→, →1)-d-Glcp-(6→, and →1)-d-Manp-(2→ residues (molar ratio of 5:4:6) and having terminal d-Glcp as side chain (degree of branching of 0.62). In vitro studies showed that PRW1 significantly induced NO production and enhanced the release of a variety of cytokines including G-CSF, GM-CSF, IL-6, IL12p40/70, MCP-1, MCP-5, MIP-1-α, MIP-2, RANTES, sTNFRI, and TNF-α. Mechanistically, PRW1 treatment triggered ERK phosphorylation to activate macrophages within 15 min and significantly increased the expression level of inducible NOS after 6 h. In summary, this study indicates that PRW1 derived from the sclerotia of P. rhinocerus is a potential immunomodulatory agent for cancer immunotherapy.
著者Liu CR, Chen JL, Chen L, Huang XS, Cheung PCK
期刊名稱Journal of Agricultural and Food Chemistry
出版年份2016
月份4
日期27
卷號64
期次16
出版社AMER CHEMICAL SOC
頁次3206 - 3214
國際標準期刊號0021-8561
電子國際標準期刊號1520-5118
語言英式英語
關鍵詞beta-mannoglucans, macrophage activation, mushroom sclerotia, Polyporus rhinocerus, polysaccharide-protein complex
Web of Science 學科類別Agriculture; Agriculture, Multidisciplinary; Chemistry; Chemistry, Applied; Food Science & Technology

上次更新時間 2021-21-02 於 23:39