Inhibition of miR-200c Restores Endothelial Function in Diabetic Mice Through Suppression of COX-2
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AbstractEndothelial dysfunction plays a crucial role in the development of diabetic vasculopathy. Our initial quantitative PCR results showed an increased miR-200c expression in arteries from diabetic mice and patients with diabetes. However, whether miR-200c is involved in diabetic endothelial dysfunction is unknown. Overexpression of miR-200c impaired endothelium-dependent relaxations (EDRs) in nondiabetic mouse aortas, whereas suppression of miR-200c by anti-miR-200c enhanced EDRs in diabetic db/db mice. miR-200c suppressed ZEB1 expression, and ZEB1 overexpression ameliorated endothelial dysfunction induced by miR-200c or associated with diabetes. More importantly, overexpression of anti-miR-200c or ZEB1 in vivo attenuated miR-200c expression and improved EDRs in db/db mice. Mechanistic study with the use of COX-2(-/-) mice revealed that COX-2 mediated miR-200c-induced endothelial dysfunction and that miR-200c upregulated COX-2 expression in endothelial cells through suppression of ZEB1 and increased production of prostaglandin E-2, which also reduced EDR. This study demonstrates for the first time to our knowledge that miR-200c is a new mediator of diabetic endothelial dysfunction and inhibition of miR-200c rescues EDRs in diabetic mice. These new findings suggest the potential usefulness of miR-200c as the target for drug intervention against diabetic vascular complications.
All Author(s) ListZhang HN, Liu J, Qu D, Wang L, Luo JY, Lau CW, Liu PS, Gao Z, Tipoe GL, Lee HK, Ng CF, Ma RCW, Yao XQ, Huang Y
Journal nameDiabetes
Volume Number65
Issue Number5
Pages1196 - 1207
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesEndocrinology & Metabolism

Last updated on 2021-19-11 at 23:52