Molecular evidence for the coordination of nitrogen and carbon metabolisms, revealed by a study on the transcriptional regulation of the agl3EFG operon that encodes a putative carbohydrate transporter in Streptomyces coelicolor
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香港中文大學研究人員

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摘要In the agl3EFGXYZ operon (SCO7167-SCO7162, abbreviated as agl3 operon) of Streptomyces coelicolor M145, agl3EFG genes encode a putative ABC-type carbohydrate transporter. The transcription of this operon has been proved to be repressed by AglR (SCO7168), a neighboring GntR-family regulator, and this repression can be released by growth on poor carbon sources. Here in this study, we prove that the transcription of agl3 operon is also directly repressed by GlnR, a central regulator governing the nitrogen metabolism in S. coelicolor. The electrophoretic mobility shift assay (EMSA) employing the agl3 promoter and mixtures of purified recombinant GlnR and Agl3R indicates that GlnR and Agl3R bind to different DNA sequences within the promoter region of agl3 operon, which is further confirmed by the DNase I footprinting assay. As Agl3R and GlnR have been demonstrated to sense the extracellular carbon and nitrogen supplies, respectively, it is hypothesized that the transcription of agl3 operon is stringently governed by the availabilities of extracellular carbon and nitrogen sources. Consistent with the hypothesis, the agl3 operon is further found to be derepressed only under the condition of poor carbon and rich nitrogen supplies, when both regulators are inactivated. It is believed that activation of the expression of agl3 operon may facilitate the absorption of extracellular carbohydrates to balance the ratio of intracellular carbon to nitrogen. (C) 2016 Elsevier Inc. All rights reserved.
著者Cen XF, Wang JZ, Zhao GP, Wang Y, Wang J
期刊名稱Biochemical and Biophysical Research Communications
出版年份2016
月份3
日期18
卷號471
期次4
出版社ACADEMIC PRESS INC ELSEVIER SCIENCE
頁次510 - 514
國際標準期刊號0006-291X
電子國際標準期刊號1090-2104
語言英式英語
關鍵詞Agl3R; Carbon metabolism; GInR; Nitrogen metabolism; Streptomyces coelicolor
Web of Science 學科類別Biochemistry & Molecular Biology; Biophysics

上次更新時間 2020-25-11 於 00:33