Molecular evidence for the coordination of nitrogen and carbon metabolisms, revealed by a study on the transcriptional regulation of the agl3EFG operon that encodes a putative carbohydrate transporter in Streptomyces coelicolor
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AbstractIn the agl3EFGXYZ operon (SCO7167-SCO7162, abbreviated as agl3 operon) of Streptomyces coelicolor M145, agl3EFG genes encode a putative ABC-type carbohydrate transporter. The transcription of this operon has been proved to be repressed by AglR (SCO7168), a neighboring GntR-family regulator, and this repression can be released by growth on poor carbon sources. Here in this study, we prove that the transcription of agl3 operon is also directly repressed by GlnR, a central regulator governing the nitrogen metabolism in S. coelicolor. The electrophoretic mobility shift assay (EMSA) employing the agl3 promoter and mixtures of purified recombinant GlnR and Agl3R indicates that GlnR and Agl3R bind to different DNA sequences within the promoter region of agl3 operon, which is further confirmed by the DNase I footprinting assay. As Agl3R and GlnR have been demonstrated to sense the extracellular carbon and nitrogen supplies, respectively, it is hypothesized that the transcription of agl3 operon is stringently governed by the availabilities of extracellular carbon and nitrogen sources. Consistent with the hypothesis, the agl3 operon is further found to be derepressed only under the condition of poor carbon and rich nitrogen supplies, when both regulators are inactivated. It is believed that activation of the expression of agl3 operon may facilitate the absorption of extracellular carbohydrates to balance the ratio of intracellular carbon to nitrogen. (C) 2016 Elsevier Inc. All rights reserved.
All Author(s) ListCen XF, Wang JZ, Zhao GP, Wang Y, Wang J
Journal nameBiochemical and Biophysical Research Communications
Year2016
Month3
Day18
Volume Number471
Issue Number4
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Pages510 - 514
ISSN0006-291X
eISSN1090-2104
LanguagesEnglish-United Kingdom
KeywordsAgl3R; Carbon metabolism; GInR; Nitrogen metabolism; Streptomyces coelicolor
Web of Science Subject CategoriesBiochemistry & Molecular Biology; Biophysics

Last updated on 2020-12-08 at 00:32