Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos
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AbstractBackground: The RNA guided CRISPR/Cas9 nucleases have been proven to be effective for gene disruption in various animal models including Xenopus tropicalis. The neural crest (NC) is a transient cell population during embryonic development and contributes to a large variety of tissues. Currently, loss-of-function studies on NC development in X. tropicalis are largely based on morpholino antisense oligonucleotide. It is worthwhile establishing targeted gene knockout X. tropicails line using CRISPR/Cas9 system to study NC development.
All Author(s) ListLiu ZZ, Cheng TTK, Shi ZY, Liu ZR, Lei Y, Wang CD, Shi WL, Chen XF, Qi XF, Cai DQ, Feng B, Deng Y, Chen YL, Zhao H
Journal nameCell and Bioscience
Detailed descriptionTo ORKTS: doi: 10.1186/s13578-016-0088-4
Year2016
Month3
Day31
Volume Number6
PublisherBIOMED CENTRAL LTD
ISSN2045-3701
LanguagesEnglish-United Kingdom
KeywordsCas9; Gene disruption; Genome editing; Multiplex deletion; Neural crest; Segmental deletion/inversion; Xenopus
Web of Science Subject CategoriesBiochemistry & Molecular Biology

Last updated on 2020-25-09 at 00:34