Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc
Publication in refereed journal


摘要A lab-on-a-disc (LOAD) is a centrifugal microfluidic set-up based on centrifugal force without using micro-pumps to drive reagents and cells to various chambers through channels and valves for reactions. A LOAD coated with conductive transparent indium tin oxide (ITO) for thermal control was developed to screen allergy-blocking agents. When the acridine orange (AO)-loaded KU-812 human basophilic cells were activated in the LOAD by stimuli, AO trapped in the cytoplasmic granules was released externally as an allergic mediator mimetic to report degranulation. This response was monitored by fluorescence when the released AO in supernatant had been transferred, with a higher spinning speed, from the reaction chamber to detection chamber in the LOAD where AO reacted with exogenous DNA. We report here the principles of the system and an improved LOAD set-up with the ITO-coated glass resistive microheater to run assays at 37 degrees C. By using this platform, we demonstrate here for the first time that triptolide, an active ingredient from the Chinese medicine herb Tripterygium wilfordii Hook f., was able to suppress the fMLP-mediated degranulation in basophils. This serves as an example how LOADs can be used to screen agents to alleviate symptoms of allergy.
著者Kwok HC, Lau PM, Wu SY, Ho HP, Gao MH, Kwan YW, Wong CK, Kong SK
詳細描述doi:10.3390/mi7030038 (Open access article).
關鍵詞acridine orange (AO); basophil activation test (BAT); bisphenol A (BPA); indium tin oxide (ITO); lab-on-a-disc (LOAD); N-formyl-methionine-leucine-phenylalanine (fMLP); phorbol 12-myristate 13-acetate (PMA); protein kinase C (PKC); Traditional Chinese Medicine (TCM); World Allergy Organization (WAO)
Web of Science 學科類別Instruments & Instrumentation; Nanoscience & Nanotechnology; Science & Technology - Other Topics

上次更新時間 2021-21-09 於 00:26