BRE facilitates skeletal muscle regeneration by promoting satellite cell motility and differentiation
Publication in refereed journal

香港中文大學研究人員

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摘要The function of the Bre gene in satellite cells was investigated during skeletal muscle regeneration. The tibialis anterior leg muscle was experimentally injured in Bre knockout mutant (BRE-KO) mice. It was established that the accompanying muscle regeneration was impaired as compared with their normal wild-type counterparts (BREWT). There were significantly fewer pax7(+) satellite cells and smaller newly formed myofibers present in the injury sites of BRE-KO mice. Bre was required for satellite cell fusion and myofiber formation. The cell fusion index and average length of newly-formed BRE-KO myofibers were found to be significantly reduced as compared with BRE-WT myofibers. It is well established that satellite cells are highly invasive which confers on them the homing ability to reach the muscle injury sites. Hence, we tracked the migratory behavior of these cells using time-lapse microscopy. Image analysis revealed no difference in directionality of movement between BRE-KO and BRE-WT satellite cells but there was a significant decrease in the velocity of BRE-KO cell movement. Moreover, chemotactic migration assays indicated that BRE-KO satellite cells were significantly less responsive to chemoattractant SDF-1 alpha than BRE-WT satellite cells. We also established that BRE normally protects CXCR4 from SDF-1 alpha-induced degradation. In sum, BRE facilitates skeletal muscle regeneration by enhancing satellite cell motility, homing and fusion.
著者Xiao LH, Lee KKH
期刊名稱Biology Open
詳細描述To ORKTS: doi: 10.1242/bio.012450
出版年份2016
月份2
日期15
卷號5
期次2
出版社COMPANY OF BIOLOGISTS LTD
頁次100 - 111
國際標準期刊號2046-6390
語言英式英語
關鍵詞BRE; Cell migration; Knockout mice; Muscle satellite cells; Skeletal muscle regeneration
Web of Science 學科類別Biology; Life Sciences & Biomedicine - Other Topics

上次更新時間 2021-16-10 於 00:26