Sonodynamic action of hypocrellin B on biofilm-producing Staphylococcus epidermidis in planktonic condition
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AbstractStaphylococcus epidermidis is an opportunistic pathogen causing biofilm-associated infections. To investigate sonodynamic action of hypocrellin B on biofilm-producing Staphylococcus epidermidis in planktonic culture, a biofilm-producing strain Staphylococcus epidermidis (ATCC 35984) was incubated with hypocrellin B and then exposed to ultrasound at intensity (I-SATA) of 1.56 W/cm(2) with a frequency of 1 MHz in continuous mode for 5 min. After sonodynamic treatment of hypocrellin B, the bacterial growth was measured using the colony counting method. Bacterial membrane integrity was investigated using a flow cytometry with propidium iodide staining. Intracellular reactive oxygen species (ROS) level was measured using a flow cytometry with DCFH-DA staining. The results showed that sonodynamic action of hypocrellin B significantly induced survival reduction of Staphylococcus epidermidis in a hypocrellin B dose-dependent manner, and a 4-log reduction was observed after the combined treatment of hypcorellin B (40 mu M) and ultrasound sonication with the intensity of 1.56 W/cm(2) for 5 min. Bacterial membrane integrity was notably damaged and the level of intracellular ROS level was remarkably increased after sonodynamic treatment. The findings demonstrated that sonodynamic action of hypocrellin B had significant antibacterial activity on biofilm-producing Staphylococcus epidermidis in planktonic condition probably through increasing intracellular ROS level to cause damage to bacterial membrane integrity. (C) 2015 Acoustical Society of America.
All Author(s) ListWang XN, Leung AW, Hua HY, Xu CS, Ip M
Journal nameJournal of the Acoustical Society of America
Year2015
Month10
Day1
Volume Number138
Issue Number4
PublisherACOUSTICAL SOC AMER AMER INST PHYSICS
Pages2548 - 2553
ISSN0001-4966
eISSN1520-8524
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesAcoustics; Audiology & Speech-Language Pathology

Last updated on 2021-12-04 at 23:31