In vitro vitamin K-2 and 1 alpha,25-dihydroxyvitamin D-3 combination enhances osteoblasts anabolism of diabetic mice
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AbstractIn this study, we evaluated the anabolic effect and the underlying cellular mechanisms involved of vitamin 1(2 (10 nM) and loc,25-dihydroxyvitamin D3 (1,25(OH)(2)D-3) (10 nM), alone and in combination, on primary osteoblasts harvested from the iliac crests of C57BL/K5J lean (+/+) and obese/diabetic (db/db) mice. A lower alkaline phosphatase (ALP) activity plus a reduced expression of bone anabolic markers and bone formation transcription factors (osteocalcin, Runx2, DIx5, ATF4 and OSX) were consistently detected in osteoblasts of db/db mice compared to lean mice. A significantly higher calcium deposits formation in osteoblasts was observed in lean mice when compared to db/db mice. Co-administration of vitamin K2 (10 nM) and 1,25(OH)(2)D-3 (10 nM) caused an enhancement of calcium deposits in osteoblasts in both strains of mice. Vitamins 1(2 and 1,25(OH)(2)D-3 co-administration time-dependently (7, 14 and 21 days) increased the levels of bone anabolic markers and bone formation transcription factors, with a greater magnitude of increase observed in osteoblasts of db/db mice. Combined vitamins 1(2 plus 1,25 (OH)(2)D-3 treatment significantly enhanced migration and the re-appearance of surface microvilli and ruffles of osteoblasts of db/db mice. Thus, our results illustrate that vitamins 1(2 plus D-3 combination could be a novel therapeutic strategy in treating diabetes-associated osteoporosis. (C) 2015 Elsevier B.V. All rights reserved.
All Author(s) ListPoon CCW, Li RWS, Seto SW, Kong SK, Ho HP, Hoi MPM, Lee SMY, Ngai SM, Chan SW, Leung GPH, Kwan YW
Journal nameEuropean Journal of Pharmacology
Year2015
Month11
Day15
Volume Number767
PublisherELSEVIER SCIENCE BV
Pages30 - 40
ISSN0014-2999
eISSN1879-0712
LanguagesEnglish-United Kingdom
Keywords1,25(OH)(2)D-3; Osteoblasts; Osteoporosis; Type 2 diabetes mellitus; Vitamin K-2
Web of Science Subject CategoriesPharmacology & Pharmacy

Last updated on 2020-14-10 at 03:12