An ultralow background substrate for protein microarray technology
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AbstractWe herein report an ultralow background substrate for protein microarrays. Conventional protein microarray substrates often suffer from non-specific protein adsorption and inhomogeneous spot morphology. Consequently, surface treatment and a suitable printing solution are required to improve the microarray performance. In the current work, we improved the situation by developing a new microarray substrate based on a fluorinated ethylene propylene (FEP) membrane. A polydopamine microspot array was fabricated on the FEP membrane, with proteins conjugated to the FEP surface through polydopamine. Uniform microspots were obtained on FEP without the application of a special printing solution. The modified FEP membrane demonstrated ultralow background signal and was applied in protein and peptide microarray analysis.
All Author(s) ListFeng H, Zhang QY, Ma HW, Zheng B
Journal nameAnalyst
Year2015
Month1
Day1
Volume Number140
Issue Number16
PublisherROYAL SOC CHEMISTRY
Pages5627 - 5633
ISSN0003-2654
eISSN1364-5528
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesChemistry; Chemistry, Analytical

Last updated on 2020-24-10 at 02:41