H2O2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization
Publication in refereed journal


Times Cited
Web of Science11WOS source URL (as at 24/11/2020) Click here for the latest count
Altmetrics Information
.

Other information
AbstractSkeletal muscles often experience oxidative stress in anaerobic metabolism and ischemia-reperfusion. This paper reports how oxidative stress affects the stiffness of cultured murine myotubes and their actin filaments polymerization dynamics. H2O2 was applied as an extrinsic oxidant to C2C12 myotubes. Atomic force microscopy results showed that short exposures to H2O2 apparently increased the stiffness of myotubes, but that long exposures made the cells softer. The turning point seemed to take place somewhere between 1 and 2 h of H2O2 exposure. We found that the stiffness change was probably due to actin filaments being favored for depolymerization after prolong H2O2 treatments, especially when the exposure duration exceeded 1 h and the exposure concentration reached 1.0 mM. Such depolymerization effect was associated with the down-regulation of thymosin beta 4, as well as the up-regulation of both cofilin2 and profilin1 after prolong H2O2 treatments.
All Author(s) ListWong SW, Sun S, Cho M, Lee KKH, Mak AFT
Journal nameAnnals of Biomedical Engineering
Year2015
Month5
Day1
Volume Number43
Issue Number5
PublisherSPRINGER
Pages1178 - 1188
ISSN0090-6964
eISSN1573-9686
LanguagesEnglish-United Kingdom
KeywordsActin filament; Cell stiffness; Cofilin2; Myotubes; Oxidative stress; Thymosin beta 4
Web of Science Subject CategoriesEngineering; Engineering, Biomedical

Last updated on 2020-25-11 at 00:00