i Antidiabetic Effect of Schisandrae Chinensis Fructus Involves Inhibition of the Sodium Glucose Cotransporter
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AbstractSchisandrae Chinensis Fructus (SCF), the fruit of Schisandra chinensis (Turcz.) Baill. (family Schisandraceae) is traditionally used as a tonic and antidiabetic agent in Asia. In this study, SCF was investigated for its effects on sodium glucose cotransporters 1 and 2 (SGLT 1 and 2) expressed in a COS-7 cell line for its specificity in inhibiting SGLT2, which is a novel mechanism to screen for potential antidiabetic agents. Using a bioassay-guided fractionation, we then tried to isolate and identify the active fraction(s)/component(s). The ethanol extract of SCF at a concentration of 1 mg/mL significantly inhibited 89% of SGLT1 and 73% of SGLT2 activities in a [C-14]-alpha-methyl-d-glucopyranoside ([C-14]-AMG) uptake assay. Fractionation of the ethanol extract yielded nine fractions, of which F8, at a concentration of 1 mg/mL, was specific in inhibiting SGLT 2 (42% inhibition, P < 0.001), without inhibiting SGLT 1. Using LC/MS-MS, three compounds, deoxyschisandrin, schisandrin B (gamma-schisandrin) and schisandrin were identified in F8 and their amounts quantified. However, subsequent evaluation in the [C-14]-AMG uptake assay showed that these three compounds failed to inhibit SGLT 2 activity indicating that the SGLT active component(s) from SCF have yet to be identified. (C) 2014 Wiley Periodicals, Inc.
All Author(s) ListQu Y, Chan JYW, Wong CW, Cheng L, Xu CS, Leung AWN, Lau CBS
Journal nameDrug Development Research
Volume Number76
Issue Number1
Pages1 - 8
LanguagesEnglish-United Kingdom
Keywordsdiabetes mellitus; Schisandrae Chinensis Fructus; SGLT; traditional Chinese medicine
Web of Science Subject CategoriesChemistry, Medicinal; Pharmacology & Pharmacy

Last updated on 2021-14-05 at 02:13