A Chinese 2-herb formula (NF3) promotes hindlimb ischemia-induced neovascularization and wound healing of diabetic rats
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AbstractDiabetic foot ulcer is closely associated with peripheral vascular disease. Enhancement of tissue oxidative stress, reduction of nitric oxide (NO) and angiogenic growth factors, and abnormal matrix metalloproteinase (MMP) activity are pathophysiological factors in post-ischemic neovascularization and diabetic wound healing. Our previous study demonstrated that the Chinese 2-herb formula, NF3, showed significant wound healing effects on diabetic foot ulcer rats. A novel rat diabetic foot ulcer with hindlimb ischemia model was established in order to strengthen our claims on the diabetic wound healing and post-ischemic neovascularization effects of NF3. Our results demonstrate that NF3 can significantly reduce the wound area of the diabetic foot ulcer rat with hindlimb ischemia by 21.6% (p < 0.05) compared with the control group. In addition, flow cytometric analysis revealed that NF3 could boost circulating EPC levels for local wound vessel incorporation. Immunohistochemical analysis showed that NF3 could significantly augment blood vessel density, VEGF and eNOS expression, and attenuate tissue oxidative stress of ischemic muscles (p < 0.001). NF3 significantly stimulated MMP activity involved in angiogenesis. Our study shows, for the first time, the beneficial effects of NF3 in wound healing and post-ischemic neovascularization in diabetes. (C) 2014 Elsevier Inc. All rights reserved.
All Author(s) ListTam JCW, Ko CH, Lau KM, To MH, Kwok HF, Chan YW, Siu WS, Etienne-Selloum N, Lau CP, Chan WY, Leung PC, Fung KP, Schini-Kerth VB, Lau CBS
Journal nameJournal of Diabetes and its Complications
Year2014
Month7
Day1
Volume Number28
Issue Number4
PublisherELSEVIER SCIENCE INC
Pages436 - 447
ISSN1056-8727
eISSN1873-460X
LanguagesEnglish-United Kingdom
KeywordsAstragalus membranaceus; Chinese medicines; Diabetic foot; Hindlimb ischemia; Rehmannia glutinosa; Wound healing
Web of Science Subject CategoriesEndocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM

Last updated on 2020-24-03 at 01:36