Methionine oxidation in albumin by fine haze particulate matter: An in vitro and in vivo study
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AbstractThe potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and)CA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes. (C) 2014 Elsevier B.V. All rights reserved.
All Author(s) ListLee KY, Wong CKC, Chuang KJ, Bien MY, Cao JJ, Han YM, Tian LW, Chang CC, Feng PH, Ho KF, Chuang HC
Journal nameJournal of Hazardous Materials
Year2014
Month6
Day15
Volume Number274
PublisherELSEVIER SCIENCE BV
Pages384 - 391
ISSN0304-3894
eISSN1873-3336
LanguagesEnglish-United Kingdom
KeywordsAir pollution; Liquid chromatography-mass spectrometry; Polycyclic aromatic hydrocarbons; Protein structure; Pulmonary
Web of Science Subject CategoriesEngineering; Engineering, Civil; ENGINEERING, CIVIL; Engineering, Environmental; ENGINEERING, ENVIRONMENTAL; Environmental Sciences; ENVIRONMENTAL SCIENCES; Environmental Sciences & Ecology

Last updated on 2021-22-07 at 01:42