Anti-inflammatory Activities of an Active Fraction Isolated from the root of Astragalus membranaceus in RAW 264.7 Macrophages
Publication in refereed journal


摘要The root of Astragalus membranaceus (AR), which has been widely used in Traditional Chinese herbal formulae for treating foot ulcer, was found to exhibit anti-inflammatory property, but its molecular mechanism still remains unknown. We previously identified the anti-inflammatory sub-fraction using bioassay-guided fractionation. The objective of the present study was to investigate the anti-inflammatory mechanism of the major active fraction (MAF) (0.039 to 0.156 mg/mL) using lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells. MAF was shown to inhibit LPS-induced mRNA and protein expression of inducible nitric oxide synthase by 54.7% and 65.1%, respectively. Additionally, MAF down-regulated the protein expression of cyclooxygenase-2 and MAPK regulator by 45.0% to 74.6%, as well as the reduction of DNA binding activity of nuclear factor kappa B (NF kappa B) by 66.5%. It also attenuated the production of prostaglandin E-2, interleukin-1 beta (IL-1 beta), IL-6 and tumor necrosis factor alpha by 21.2% to 86.2%. Furthermore, the chemical constituents of MAF were identified. A total of 13 known chemical compounds were found in MAF, including five isoflavonoids and eight saponins. In conclusion, a bioactive fraction of AR was identified which possessed anti-inflammatory property by reducing the release of inflammatory mediators and inactivation of NF kappa B through MAPK signalling pathway. Copyright (c) 2013 John Wiley & Sons, Ltd.
著者Lai PKK, Chan JYW, Wu SB, Cheng L, Ho GKW, Lau CP, Kennelly EJ, Leung PC, Fung KP, Lau CBS
期刊名稱Phytotherapy Research
頁次395 - 404
關鍵詞anti-inflammatory; Astragalus membranaceus; macrophages; Traditional Chinese Medicine
Web of Science 學科類別Chemistry, Medicinal; CHEMISTRY, MEDICINAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

上次更新時間 2021-19-01 於 01:23