Anti-inflammatory Activities of an Active Fraction Isolated from the root of Astragalus membranaceus in RAW 264.7 Macrophages
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摘要The root of Astragalus membranaceus (AR), which has been widely used in Traditional Chinese herbal formulae for treating foot ulcer, was found to exhibit anti-inflammatory property, but its molecular mechanism still remains unknown. We previously identified the anti-inflammatory sub-fraction using bioassay-guided fractionation. The objective of the present study was to investigate the anti-inflammatory mechanism of the major active fraction (MAF) (0.039 to 0.156 mg/mL) using lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells. MAF was shown to inhibit LPS-induced mRNA and protein expression of inducible nitric oxide synthase by 54.7% and 65.1%, respectively. Additionally, MAF down-regulated the protein expression of cyclooxygenase-2 and MAPK regulator by 45.0% to 74.6%, as well as the reduction of DNA binding activity of nuclear factor kappa B (NF kappa B) by 66.5%. It also attenuated the production of prostaglandin E-2, interleukin-1 beta (IL-1 beta), IL-6 and tumor necrosis factor alpha by 21.2% to 86.2%. Furthermore, the chemical constituents of MAF were identified. A total of 13 known chemical compounds were found in MAF, including five isoflavonoids and eight saponins. In conclusion, a bioactive fraction of AR was identified which possessed anti-inflammatory property by reducing the release of inflammatory mediators and inactivation of NF kappa B through MAPK signalling pathway. Copyright (c) 2013 John Wiley & Sons, Ltd.
著者Lai PKK, Chan JYW, Wu SB, Cheng L, Ho GKW, Lau CP, Kennelly EJ, Leung PC, Fung KP, Lau CBS
期刊名稱Phytotherapy Research
詳細描述doi:10.1002/ptr.5002.
出版年份2014
月份3
日期1
卷號28
期次3
出版社WILEY-BLACKWELL
頁次395 - 404
國際標準期刊號0951-418X
電子國際標準期刊號1099-1573
語言英式英語
關鍵詞anti-inflammatory; Astragalus membranaceus; macrophages; Traditional Chinese Medicine
Web of Science 學科類別Chemistry, Medicinal; CHEMISTRY, MEDICINAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

上次更新時間 2021-19-01 於 01:23