Molecular identification and cytotoxicity study of herbal medicinal materials that are confused by Aristolochia herbs
Publication in refereed journal


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摘要Herbal materials derived from Aristolochia species contain carcinogenic aristolochic acids (AAs) and have been used as traditional Chinese medicines (TCMs) or adulterants of other TCMs. The purpose of this study is to identify the TCMs Stephaniae Tetrandrae Radix, Akebiae Caulis, Aucklandia Radix and Aristolochiae Fructus by sequencing the matK, rbcL, trnH-psbA and tmL-tmF DNA regions. The cytotoxicities of AAs and these TCMs were also studied in COS-7 and HEK-293 cells. Diagnostic polymorphic sites were identified in all the four DNA loci for the differentiation of genuine herbs from their adulterants/substitute. The 48 h IC50 of AAI were 78 mu M (COS-7) and 70 mu M (HEK-293) while the IC50 of AAII were higher than 100 mu M in both cell lines. Except Aucklandia Radix, cytotoxicity study also showed that AA-containing herbs were more toxic than their corresponding genuine herbs and substitute. (C) 2013 Elsevier Ltd. All rights reserved.
著者Li M, Au KY, Lam H, Cheng L, But PPH, Shaw PC
期刊名稱Food Chemistry
出版年份2014
月份3
日期15
卷號147
出版社ELSEVIER SCI LTD
頁次332 - 339
國際標準期刊號0308-8146
電子國際標準期刊號1873-7072
語言英式英語
關鍵詞Akebia; Aristolochia; Aristolochic acid; Aucklandia lappa; Cardiocrinum giganteum var. yunnanense; Stephania tetrandra
Web of Science 學科類別Chemistry; Chemistry, Applied; CHEMISTRY, APPLIED; Food Science & Technology; FOOD SCIENCE & TECHNOLOGY; Nutrition & Dietetics; NUTRITION & DIETETICS

上次更新時間 2020-18-10 於 01:19