A pneumatic valve controlled microdevice for bioanalysis
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AbstractThis paper describes a pneumatic valve controlled microdevice for performing mixing and reaction. This microdevice combined the degassed polydimethylsiloxane (PDMS) pumping method with a syringe-actuated valve system to control the dispensing and mixing of nanoliter solutions. The syringe was used to manually generate vacuum and to open the valves. Upon the opening of the valve, the microchamber was filled with the solution, which was driven by the external atmosphere through the degassed PDMS microchannel. With this microdevice, the enzymatic kinetics of alkaline phosphatase converting the fluorescein diphosphate was studied, and the Michaelis-Menten kinetics was analyzed. The microdevice has the advantages of simplicity and low cost in fabrication and operation. (C) 2013 AIP Publishing LLC.
All Author(s) ListZhou XH, Zhou XC, Zheng B
Journal nameBiomicrofluidics
Year2013
Month9
Day1
Volume Number7
Issue Number5
PublisherAMER INST PHYSICS
ISSN1932-1058
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesBiochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; BIOPHYSICS; Nanoscience & Nanotechnology; NANOSCIENCE & NANOTECHNOLOGY; Physics; Physics, Fluids & Plasmas; PHYSICS, FLUIDS & PLASMAS; Science & Technology - Other Topics

Last updated on 2020-30-06 at 05:59