IL-15 and macrophage secretory factors facilitate immune activation of neonatal natural killer cells by lipoteichoic acid
Publication in refereed journal


引用次數
替代計量分析
.

其它資訊
摘要Neonates possess a relatively "naive", yet inducible immune system. Our hypothesis is that upon strategic antigen exposure, cytokine priming and sensitization by accessory cells, natural killer (NK) cells could be activated to become a functional phenotype. We investigated the in vitro stimulation of cord blood (CB) and adult NK cells upon challenge with lipoteichoic acid (LTA), interleukin (IL)-15 and LTA-primed autologous macrophage-conditioned medium, using CD107a and CD69 phenotypes as indicators of activation. We also examined response of CB macrophages to LTA, in terms of P44/42 extracellular signal-regulated kinases (ERK1/2) activation and cytokine secretion. LTA significantly induced secretion of inflammatory cytokines tumor necrotic factor (TNF)-alpha, IL-6, IL-12 and activated the upstream signal of ERK1/2 phosphorylation in neonatal macrophages. The magnitude of responses to stimulation differed between neonatal and adult NK cells. Co-stimulation with IL-15 was critical for expansion of the CD69 and CD107a NK subpopulations in both neonatal and adult cells, upon a LTA challenge. NK cell activation could be enhanced by LTA-primed autologous macrophages through secretory factors. Our results indicated that neonatal macrophages and NK cells can evoke immunologic responses to a Gram-positive bacterial antigen. The combinatory priming strategy is relevant for development of novel protocols, such as IL-15 treatment, to compensate for the immaturity of the innate immune system in newborns against bacterial infections. (c) 2012 Elsevier Ltd. All rights reserved.
著者Cheng SF, Ho JWY, Chan KYY, Leung TY, Lam HS, Fong ON, Li KR, Ng PC
期刊名稱Cytokine
出版年份2013
月份2
日期1
卷號61
期次2
出版社ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
頁次499 - 505
國際標準期刊號1043-4666
電子國際標準期刊號1096-0023
語言英式英語
關鍵詞IL-15; Lipoteichoic acid; Macrophages; Natural killer cells; Neonatal blood
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY; Immunology; IMMUNOLOGY

上次更新時間 2020-20-09 於 03:00