Identification of Baiying (Herba Solani Lyrati) commodity and its toxic substitute Xungufeng (Herba Aristolochiae Mollissimae) using DNA barcoding and chemical profiling techniques
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摘要Baiying derived from Solanum lyratum Hance is a commonly consumed natural product for ethnomedical treatment of cancer. One of the substitutes present in the market is a carcinogenic aristolochic acids-containing herb Xungufeng derived from Aristolochia mollissima Thunb. The purpose of this study is to establish DNA barcodes, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and cytotoxicity assay to differentiate Baiying from Xungufeng. A total of 30 DNA sequences from five DNA barcodes (ITS, matK, rbcL, trnH-psbA and trnL-trnF) were generated to differentiate S. lyratum from A. mollissima and authenticate ten samples of Baiying and Xungufeng commodities. Using aristolochic acids as standard markers, TLC and HPLC analyses also successfully authenticated these commodities. In vitro cytotoxicity assay using HEK-293 and Vero cells demonstrated that Xungufeng was significantly more toxic than Baiying. This is the first study applying an integrated molecular, chemical and biological approach to differentiate traditional Chinese medicine from Aristolochia adulterant. (C) 2012 Elsevier Ltd. All rights reserved.
著者Li M, Au KY, Lam H, Cheng L, Jiang RW, But PPH, Shaw PC
期刊名稱Food Chemistry
詳細描述DOI: 10.1016/j.foodchem.2012.06.049.
出版年份2012
月份12
日期1
卷號135
期次3
出版社ELSEVIER SCI LTD
頁次1653 - 1658
國際標準期刊號0308-8146
電子國際標準期刊號1873-7072
語言英式英語
關鍵詞Aristolochia mollissima; Aristolochic acids; Authentication; DNA barcoding; Solanum lyratum
Web of Science 學科類別Chemistry; Chemistry, Applied; CHEMISTRY, APPLIED; Food Science & Technology; FOOD SCIENCE & TECHNOLOGY; Nutrition & Dietetics; NUTRITION & DIETETICS

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