EXPRESSION AND ASSOCIATION OF TRPC1 WITH TRPC3 DURING SKELETAL MYOGENESIS
Publication in refereed journal

香港中文大學研究人員

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摘要Introduction: TRPC1 and TRPC3 proteins are widely expressed in skeletal muscles in forming calcium-permeable channels. Herein we characterize the expression pattern of TRPC transcripts during skeletal myogenesis in C2C12 myoblasts. Methods: We used polymerase chain reaction and Western blotting to detect expression levels, immunohistochemistry for subcellular localization, and co-immunoprecipitation techniques to assess interaction. Results: TRPC1 localizes to the cytoplasm and is enriched in the perinuclear region in undifferentiated myoblasts. Expression of TRPC1 increases significantly during myogenesis and resides mainly in differentiated myocytes and myotubes. TRPC3 is absent in undifferentiated myoblasts, is dramatically upregulated in differentiated culture, and is preferentially expressed in myotubes. Physical interaction of TRPC1 TRPC3 was observed, suggesting the possible existence of heteromers. Conclusions: Expression of TRPC1 and TRPC3 is tightly regulated during myogensis. Evidence of TRPC1 TRPC3 interaction was first demonstrated in a muscle cell line. The functional consequences of this interaction remain to be established. Muscle Nerve 44: 358-365, 2011
著者Cheung KK, Yeung SS, Au SW, Lam LS, Dai ZQ, Li YH, Yeung EW
期刊名稱Muscle and Nerve
出版年份2011
月份9
日期1
卷號44
期次3
出版社WILEY-BLACKWELL
頁次358 - 365
國際標準期刊號0148-639X
語言英式英語
關鍵詞myogenesis; skeletal muscle; store-operated channel; TRPC1; TRPC3
Web of Science 學科類別Clinical Neurology; CLINICAL NEUROLOGY; Neurosciences; NEUROSCIENCES; Neurosciences & Neurology

上次更新時間 2020-20-10 於 02:21