A potent antitumor polysaccharide from the edible brown seaweed Hydroclathrus clathratus
Publication in refereed journal


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摘要A sulfated polysaccharide H3-a1 [designated polysaccharide fraction, H3-a1, of Hydroclathrus clathratus (HCP)] was isolated and purified from an edible brown seaweed Hydroclathrus clathratus; HCP contained about 8.6% protein and a relatively high sulfur content (8.2%). HCP significantly affected the growth of human acute promyelocytic leukemia cells (HL-60), human breast carcinoma (MCF-7) and human hepatocellular carcinoma cancer cell lines. As revealed by flow cytometry, when HCP was co-incubated with HL-60 and MCF-7 cells for 72 h, it induced significant sub-G1 arrest (apoptosis) of these cells. HCP caused G1 phase arrest but did not induce terminal differentiation of these cancer cells. In vivo antitumor tests showed that HCP inhibited tumor growth at doses of 20 and 50 mg kg(-1) in tumor-bearing BALB/c (supplied by the Laboratory Animal Services Centre of The Chinese University of Hong Kong) mice, but this effect was not due to an in vivo inhibitory effect on tumorigenesis. Nevertheless, HCP suppressed ascitic Sarcoma 180 tumor growth and prolonged the life span of the tumor-bearing mice by about 30-40%. HCP had no observed toxic side effects; it did not affect blood cells, or body, liver or spleen weights of mice 4, 7 and 10 days after treatment. This sulfated polysaccharide induced increased tumor necrosis factor-alpha (TNF-alpha) level in mouse serum. It also activated peritoneal macrophages to secrete TNF-alpha and nitric oxide.
著者Wang H, Chiu LCM, Ooi VEC, Ang PO
期刊名稱Botanica Marina
出版年份2010
月份6
日期1
卷號53
期次3
出版社WALTER DE GRUYTER & CO
頁次265 - 274
國際標準期刊號0006-8055
電子國際標準期刊號1437-4323
語言英式英語
關鍵詞antitumor; apoptosis; Hydroclathrus clathratus; seaweed; sulfated polysaccharide
Web of Science 學科類別Marine & Freshwater Biology; MARINE & FRESHWATER BIOLOGY; Plant Sciences; PLANT SCIENCES

上次更新時間 2021-09-04 於 00:10