Cyclooxygenase-2-Derived Prostaglandin F-2 alpha Mediates Endothelium-Dependent Contractions in the Aortae of Hamsters With Increased Impact During Aging
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AbstractHypertension and vascular dysfunction result in the increased release of endothelium-derived contracting factors (EDCFs), whose identity is poorly defined. We tested the hypothesis that endothelial cyclooxygenase (COX)-2 can generate EDCFs and identified the possible EDCF candidate. Changes in isometric tension of aortae of young and aged hamsters were recorded on myograph. Real-time changes in intracellular calcium concentrations ([Ca2+](i)) in native aortic endothelial cells were measured by imaging. Endothelium-dependent contractions were triggered by acetylcholine (ACh) after inhibition of nitric oxide production and they were abolished by COX-2 but not COX-1 inhibitors or by thromboxane-prostanoid receptor antagonists. 2-Aminoethoxydiphenyl borate (cation channel blocker) eliminated endothelium-dependent contractions and ACh-stimulated rises in endothelial cell [Ca2+](i). RT-PCR and Western blotting showed COX-2 expression mainly in the endothelium. Enzyme immunoassay and high-performance liquid chromatography-coupled mass spectrometry showed release of prostaglandin (PG)F-2 alpha and prostacyclin (PGI(2)) increased by ACh; only PGF(2 alpha) caused contraction at relevant concentrations. COX-2 expression, ACh-stimulated contractions, and vascular sensitivity to PGF(2 alpha) were augmented in aortae from aged hamsters. Human renal arteries also showed thromboxane-prostanoid receptor-mediated ACh- or PGF(2 alpha)-induced contractions and COX-2-dependent release of PGF(2 alpha). The present study demonstrates that PGF(2 alpha), derived from COX-2, which is localized primarily in the endothelium, is the most likely EDCF underlying endothelium-dependent, thromboxane-prostanoid receptor-mediated contractions to ACh in hamster aortae. These contractions involved increases in endothelial cell [Ca-2 alpha] i. The results support a critical role of COX-2 in endothelium-dependent contractions in this species with an increased importance during aging and, possibly, a similar relevance in humans. (Circ Res. 2009; 104: 228-235.)
All Author(s) ListWong SL, Leung FP, Lau CW, Au CL, Yung LM, Yao XQ, Chen ZY, Vanhoutte PM, Gollasch M, Huang Y
Journal nameCirculation Research
Volume Number104
Issue Number2
Pages228 - 235
LanguagesEnglish-United Kingdom
Keywordsaging; aorta; cyclooxygenase-2; endothelium-derived contracting factors; thromboxane-prostanoid receptor
Web of Science Subject CategoriesCardiac & Cardiovascular Systems; CARDIAC & CARDIOVASCULAR SYSTEMS; Cardiovascular System & Cardiology; Hematology; HEMATOLOGY; Peripheral Vascular Disease; PERIPHERAL VASCULAR DISEASE

Last updated on 2021-28-02 at 00:52