Analysis of bone marrow fatty acid composition using high-resolution proton NMR spectroscopy
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摘要High-resolution (1)H NMR spectroscopy as a complementary method in the analysis of human bone marrow fatty acid (FA) composition was examined. Marrow FA composition in 10 bone samples measured by, NMR and gas chromatography (GC) were compared. NMR T(1) relaxation time of FA was determined and reproducibility tests were performed to assess the variability. Good correlations were obtained between the NMR and GC results for omega-6 polyunsaturated fatty acid (PUFA) (Spearman r, 0.878), omega-3 PUFA (0.895), monounsatu rated FA (0.964) and saturated FA (0.939). The NMR method tended to overestimate saturated FA and underestimate omega-3/omega-6 ratio compared to GC results. T, relaxation time of marrow FA was 0.56-3.65 s. Coefficient of variation of the NMR method was 0.6-8.2% in intra-experimental and 0.2-8.4% in inter-experimental measurements. This study demonstrates a complernentary role for (1)H NMR spectroscopy as an additional analytical tool in human lipid research. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
著者Yeung DKW, Lam SL, Griffith JF, Chan ABW, Chen ZY, Tsang PH, Leung PC
期刊名稱Chemistry and Physics of Lipids
出版年份2008
月份2
日期1
卷號151
期次2
出版社ELSEVIER IRELAND LTD
頁次103 - 109
國際標準期刊號0009-3084
語言英式英語
關鍵詞(1)H NMR spectroscopy; bone marrow; fatty acids; osteoporosis
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

上次更新時間 2020-27-11 於 01:39