Analysis of bone marrow fatty acid composition using high-resolution proton NMR spectroscopy
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AbstractHigh-resolution (1)H NMR spectroscopy as a complementary method in the analysis of human bone marrow fatty acid (FA) composition was examined. Marrow FA composition in 10 bone samples measured by, NMR and gas chromatography (GC) were compared. NMR T(1) relaxation time of FA was determined and reproducibility tests were performed to assess the variability. Good correlations were obtained between the NMR and GC results for omega-6 polyunsaturated fatty acid (PUFA) (Spearman r, 0.878), omega-3 PUFA (0.895), monounsatu rated FA (0.964) and saturated FA (0.939). The NMR method tended to overestimate saturated FA and underestimate omega-3/omega-6 ratio compared to GC results. T, relaxation time of marrow FA was 0.56-3.65 s. Coefficient of variation of the NMR method was 0.6-8.2% in intra-experimental and 0.2-8.4% in inter-experimental measurements. This study demonstrates a complernentary role for (1)H NMR spectroscopy as an additional analytical tool in human lipid research. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
All Author(s) ListYeung DKW, Lam SL, Griffith JF, Chan ABW, Chen ZY, Tsang PH, Leung PC
Journal nameChemistry and Physics of Lipids
Volume Number151
Issue Number2
Pages103 - 109
LanguagesEnglish-United Kingdom
Keywords(1)H NMR spectroscopy; bone marrow; fatty acids; osteoporosis
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

Last updated on 2021-19-09 at 00:55