High-level gentamicin resistance mediated by a Tn4001-like transposon in seven nonclonal hospital isolates of Streptococcus pasteurianus
Publication in refereed journal


Times Cited
Web of Science9WOS source URL (as at 15/01/2021) Click here for the latest count
Altmetrics Information
.

Other information
AbstractWe report on the first occurrence of high-level gentamicin resistance (MICs >= 512 mu g/ml) in seven clinical isolates of Streptococcus pasteurianus from Hong Kong. These seven isolates were confirmed to be the species S. pasteurianus on the basis of nucleotide sequencing of the superoxide dismutase (sodA) gene. Epidemiological data as well as the results of pulse-field gel electrophoresis analysis suggested that the seven S. pasteurianus isolates did not belong to the same clone. Molecular characterization showed that they carried a chromosomal, transposon-borne resistance gene [aac(6')Ie-aph(2 '')Ia] which was known to encode a bifunctional aminoglycoside-modifying enzyme. The genetic arrangement of this transposon was similar to that of Tn4001, a transposon previously recovered from Staphylococcus aureus and other gram-positive isolates. Genetic linkage with other resistance elements, such as the ermB gene for erythromycin resistance, was not evident. On the basis of these findings, we suggest that routine screening for high-level gentamicin resistance should be recommended for all clinically significant blood culture isolates. This is to avoid the inadvertent use of short-course combination therapy with penicillin and gentamicin, which may lead to the failure of treatment for endocarditis, the selection of drug-resistant Streptococcus pasteurianus and other gram-positive organisms, as well as the unnecessary usage of gentamicin, a drug with potential toxicity.
All Author(s) ListChow VCY, Hawkey PM, Chan EWC, Chin ML, Au TK, Fung DKC, Chan RCY
Journal nameAntimicrobial Agents and Chemotherapy
Year2007
Month7
Day1
Volume Number51
Issue Number7
PublisherAMER SOC MICROBIOLOGY
Pages2508 - 2513
ISSN0066-4804
eISSN1098-6596
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesMicrobiology; MICROBIOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2021-15-01 at 23:54