Identification of PEG10 as a progression related biomarker for hepatocellular carcinoma
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摘要Widespread DNA copy number alterations are well recognized in hepatocellular carcinoma (HCC), although the affected genes expression remained largely undefined. In this study, we performed genome-wide analysis on HCC to examine the relationship between gene copy number and corresponding transcriptional changes. To ensure analysis on a homogenous population of tumor cells, integrative analysis of array-based CGH and expression profilings was performed on 20 HCC cell lines using a 19,200-element cDNA microarray platform. Further validation studies were carried out on a large series of primary HCC tumors and paired adjacent non-malignant liver to ascertain finding. Correlative analyses highlighted 31 candidate genes that manifested both copy gains and gene up-regulations (R-2 > 0.5; p < 0.05). Of interest was over-expressed paternally expressed 10 (PEG10) resided within the chromosome region 7q21 that has been implicated in the progression of HCC. Quantitative PCR and qRT-PCR studies verified concurrent genomic gains and over-expression of PEG10 in HCC cell lines and primary tumors (34/40 cases; 85%). In addition, qRT-PCR demonstrated a significant progressive trend of increasing PEG10 expressions from the putative pre-malignant adjacent livers to early resectable HCC tumors, and to late inoperable HCCs (p = 0.007). In summary, the present study demonstrated the usefulness of integrated genomic and expression profilings in identifying candidate genes within regions of genomic alteration. Our results also suggested that PEG10 may be a potential biomarker in the progressive development of HCC, and that genomic gain represents one of the major mechanisms in the induction of PEG10 over-expressions. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
著者Ip WK, Lai PBS, Wong NLY, Sy SMH, Beheshti B, Squire JA, Wong N
期刊名稱Cancer Letters
出版年份2007
月份6
日期8
卷號250
期次2
出版社ELSEVIER IRELAND LTD
頁次284 - 291
國際標準期刊號0304-3835
電子國際標準期刊號1872-7980
語言英式英語
關鍵詞array-based CGH; cDNA microarray; hepatocellular carcinoma; PEG10
Web of Science 學科類別Oncology; ONCOLOGY

上次更新時間 2020-20-10 於 01:56