Non-starch polysaccharides from different developmental stages of Pleurotus tuber-regium inhibited the growth of human acute promyelocytic leukemia HL-60 cells by cell-cycle arrest and/or apoptotic induction
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AbstractThe chemical compositions and in vitro anti-proliferative activities of the water-soluble non-starch polysaccharides (NSPs) extracted from the fruiting body, mycelium and culture medium (coded as HWE, EDP and CEP, respectively) of a novel edible mushroom Pleurotus tuber-regium (PTR) were compared. HWE is likely to be a heteropolysaccharide-protein complex with a high molecular weight of 186 x 10(4). Glucose-rich EDP had a moderate MW of 50.9 X 10(4) while mannose-rich CEP had a low MW of 4.4 x 10(4). Among all PTR NSPs, HWE had the strongest cytotoxicity (approximate IC50:25 mu g/ml) and exerted effective anti-proliferative activity at 200 mu g/ml against human acute promyelocytic leukemia cells (HL-60). All PTR NSPs induced apoptosis in HL-60 cells with an increase in the ratio of Bax/Bcl-2. Analysis from flow cytometry and Western blot demonstrated that EDP caused G(2)/M arrest in HL-60 cells by lowering the Cdk1 expression while HWE caused S arrest in the HL-60 cells by a depletion of Cdk2 and an increase in cyclin E expression. (c) 2006 Elsevier Ltd. All rights reserved.
All Author(s) ListWong SM, Wong KK, Chiu LCM, Cheung PCK
Journal nameCarbohydrate Polymers
Volume Number68
Issue Number2
Pages206 - 217
LanguagesEnglish-United Kingdom
Keywordsapoptosis; cell-cycle arrest; HL-60 cells; mushroom sclerotia; non-starch polysaccharides; Pleurotus tuber-regium
Web of Science Subject CategoriesChemistry; Chemistry, Applied; CHEMISTRY, APPLIED; Chemistry, Organic; CHEMISTRY, ORGANIC; Polymer Science; POLYMER SCIENCE

Last updated on 2020-08-07 at 03:46