Hypermethylation of RASSF1A in human and rhesus placentas
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摘要The pseudomalignant nature of the placenta prompted us to search for tumor suppressor gene hypermethylation, a phenomenon widely reported in cancer, in the human placenta. Nine tumor suppressor genes were studied. Hypermethylation of the Ras association domain family 1 A (RASSF1A) gene was found in human placentas from all three trimesters of pregnancy but was absent in other fetal tissues. Hypermethylation of Rassf1 was similarly observed in placentas from the rhesus monkey but not the mouse. An. inverse relationship between RASSF1A promoter methylation and gene expression was demonstrated by bisulfite sequencing of microdissected placental cells and immunohistochemical staining of placental tissue sections using an anti-RASSF1A antibody. Treatment of choriocarcinoma cell lines, JAR and JEG3, by 5-aza-2'-deoxycytidine and trichostatin A led to reduction in RASSF1A methylation but increased expression. These observations extend the analogy between the primate placenta and malignant tumors to the epigenetic level.
著者Chiu RWK, Chim SSC, Wong IHN, Wong CSC, Lee WS, To KF, Tong JHM, Yuen RKC, Shum ASW, Chan JKC, Chan LYS, Yuen JWF, Tong YK, Weier JF, Ferlatte C, Leung TN, Lau TK, Lo KW, Lo YMD
期刊名稱American Journal of Pathology
出版年份2007
月份3
日期1
卷號170
期次3
出版社AMER SOC INVESTIGATIVE PATHOLOGY, INC
頁次941 - 950
國際標準期刊號0002-9440
語言英式英語
Web of Science 學科類別Pathology; PATHOLOGY

上次更新時間 2020-21-11 於 00:27