Dual effect of endothelin 1 on angiotensin II-potentiated purinergic neurotransmission in prostatic rat vas deferens
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摘要Angiotensin and endothelin are vasoactive peptides with neuromodulatory effect, however their interactions in facilitating neurotransmission are largely unknown. In the present study, effort was made to examine how endothelin I modulates angiotensin II-potentiated purinergic neurotransmission in prostatic rat vas deferens. Both peptides facilitated field-stimulated muscle contraction in a concentration-dependent manner with K-d values of 16.97 +/- 6.47 and 2.46 +/- 0.83 nM for angiotensin II and endothelin I, respectively. Hill plot analysis gave Hill constants of 0.91 0.15 and 0.97 +/- 0.26 for angiotensin II and endothelin I, respectively. Correlation analysis indicated that the extent of potentiation by angiotensin 11, but not endothelin 1, was proportional to the basal field-stimulated muscle contraction. In the presence of low concentrations of endothelin (<= 3 nM), angiotensin II-potentiated field-stimulated contraction was further enhanced by endothelin. However, in the presence of high concentrations of endothelin 1 ( >= 10 nM), a much increased basal field-stimulated contraction was observed, and the addition of angiotensin 11 did not elicit any further enhancement in the contractile response. Intriguingly, after prolonged exposure of prostatic rat vas deferens to a high concentration of endothelin 1, the addition of angiotensin II induced a refractory response to field-stimulation. Taken together, our result indicated that endothelin 1 augmented angiotensin II-facilitated purinergic neurotransmission in prostatic rat vas deferens at low concentrations, but inhibited gradually at high concentrations. (c) 2006 Elsevier B.V. All rights reserved.
著者Cheng M, Lee SST, Cheung WT
期刊名稱European Journal of Pharmacology
出版年份2007
月份2
日期28
卷號557
期次2-3
出版社ELSEVIER SCIENCE BV
頁次204 - 211
國際標準期刊號0014-2999
電子國際標準期刊號1879-0712
語言英式英語
關鍵詞angiotensin; endothelin; purinergic neurotransmission; rat vas deterens
Web of Science 學科類別Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

上次更新時間 2020-20-10 於 01:46