Dual effect of endothelin 1 on angiotensin II-potentiated purinergic neurotransmission in prostatic rat vas deferens
Publication in refereed journal


Times Cited
Web of Science0WOS source URL (as at 19/09/2020) Click here for the latest count
Altmetrics Information
.

Other information
AbstractAngiotensin and endothelin are vasoactive peptides with neuromodulatory effect, however their interactions in facilitating neurotransmission are largely unknown. In the present study, effort was made to examine how endothelin I modulates angiotensin II-potentiated purinergic neurotransmission in prostatic rat vas deferens. Both peptides facilitated field-stimulated muscle contraction in a concentration-dependent manner with K-d values of 16.97 +/- 6.47 and 2.46 +/- 0.83 nM for angiotensin II and endothelin I, respectively. Hill plot analysis gave Hill constants of 0.91 0.15 and 0.97 +/- 0.26 for angiotensin II and endothelin I, respectively. Correlation analysis indicated that the extent of potentiation by angiotensin 11, but not endothelin 1, was proportional to the basal field-stimulated muscle contraction. In the presence of low concentrations of endothelin (<= 3 nM), angiotensin II-potentiated field-stimulated contraction was further enhanced by endothelin. However, in the presence of high concentrations of endothelin 1 ( >= 10 nM), a much increased basal field-stimulated contraction was observed, and the addition of angiotensin 11 did not elicit any further enhancement in the contractile response. Intriguingly, after prolonged exposure of prostatic rat vas deferens to a high concentration of endothelin 1, the addition of angiotensin II induced a refractory response to field-stimulation. Taken together, our result indicated that endothelin 1 augmented angiotensin II-facilitated purinergic neurotransmission in prostatic rat vas deferens at low concentrations, but inhibited gradually at high concentrations. (c) 2006 Elsevier B.V. All rights reserved.
All Author(s) ListCheng M, Lee SST, Cheung WT
Journal nameEuropean Journal of Pharmacology
Year2007
Month2
Day28
Volume Number557
Issue Number2-3
PublisherELSEVIER SCIENCE BV
Pages204 - 211
ISSN0014-2999
eISSN1879-0712
LanguagesEnglish-United Kingdom
Keywordsangiotensin; endothelin; purinergic neurotransmission; rat vas deterens
Web of Science Subject CategoriesPharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-20-09 at 01:01