Cell-cycle arrest and apoptosis induction in human breast carcinoma MCF-7 cells by carboxymethylated beta-glucan from the mushroom sclerotia of Pleurotus tuber-regium
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AbstractThe mechanism for the anti-tumor activity of a water-soluble carboxymethylated P-glucan (CMPTR), partially synthesized from an insoluble native glucan isolated from the sclerotia of Pleurotus tuber-regium, was studied using human breast carcinoma MCF-7 breast cancer cells in vitro. CMPTR-induced anti-proliferative activity dose-dependently, with an IC50 of 204 mu g/ml. CMPTR inhibited the cell proliferation of MCF-7 by arresting the G, phase of its cell cycle after 48 h of incubation as shown by flow cytometry. Such G, phase arrest was associated with the down-regulation of cyclin D I and cyclin E expressions in the breast cancer cells. In addition, the CMPTR-treated MCF-7 cancer cells-were associated with decreased expression of anti-apoptotic Bcl-2 protein and increased expression of Bax/ Bcl-2 ratio. This study shows that CMPTR can inhibit the proliferation of MCF-7 by cell-cycle arrest and apoptosis induction. The potential development of this mushroom polysaccharide as a water-soluble anti-tumor agent requires further investigation. (c) 2006 Elsevier Ltd. All rights reserved.
All Author(s) ListZhang M, Cheung PCK, Chiu LCM, Wong EYL, Ooi VEC
Journal nameCarbohydrate Polymers
Volume Number66
Issue Number4
Pages455 - 462
LanguagesEnglish-United Kingdom
Keywordsapoptosis; beta-glucan; carboxymethylation; cell-cycle arrest; MCF-7 cells; mushroom sclerotia; Pleurotus tuber-regium; sclerotia
Web of Science Subject CategoriesChemistry; Chemistry, Applied; CHEMISTRY, APPLIED; Chemistry, Organic; CHEMISTRY, ORGANIC; Polymer Science; POLYMER SCIENCE

Last updated on 2020-04-07 at 03:25