Determination of aflatoxins in Chinese medicinal herbs by high-performance liquid chromatography using immunoaffinity column cleanup - Improvement of recovery
Publication in refereed journal

香港中文大學研究人員

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摘要Although analytical methods are available for the determination of aflatoxins in medicinal herbs, none of them can be applied satisfactorily to all sample matrices. The difficulty arises from the complex chemical composition of the herbs. Recovery is generally low by using immunoaffinity column cleanup due to the acidity of the water extractive leading to a weakened binding affinity. As a solvent for dilution and neutralization, phosphate buffer saline is useful for certain herbs but not for others that have high acidity. The problem can be solved by using 0.1 M phosphate buffer, which has a higher buffering capacity and eliminates sodium chloride. The modified method was validated by the analysis of a certified reference material and shown to be useful for the determination of aflatoxins in herbal samples of high acidity. (c) 2006 Elsevier B.V. All rights reserved.
著者Ip SP, Che CT
期刊名稱Journal of Chromatography A
出版年份2006
月份12
日期1
卷號1135
期次2
出版社ELSEVIER SCIENCE BV
頁次241 - 244
國際標準期刊號0021-9673
電子國際標準期刊號1873-3778
語言英式英語
關鍵詞aflatoxins; herbal medicine; immunoaffinity column
Web of Science 學科類別Biochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL

上次更新時間 2021-15-01 於 23:28