In vitro immunomodulatory activities of a newly concocted traditional Chinese medicine formula: VI-28
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摘要Previous studies have suggested that Vigconic VI-28, an anti-aging traditional Chinese medicine (TCM) formula containing Radix Ginseng and Cornu Cervi Pantotrichum, possesses immunological efficacy. This in vitro study further investigated the immunomodulatory effects of the hot water extracts of VI-28. The study included (1) colorimetric 5-bromo-2'-deoxy-uridine proliferation ELISA for estimating mitogenicity in human peripheral blood mononuclear cells (PBMC), (2) immunufluorescence staining for measuring the expression of IL-2 receptor alpha (CD25) on lymphocytes, (3) cytometric bead array (CBA) for quantifying cytokine liberation from PBNIC, and (4) intracellular immunophenotyping for macrophage phagocytosis and hydrogen peroxide (H2O2) production from monocytes. The results demonstrated that VI-28 (1) could dose-dependently inhibited the proliferation of unstimulated and lipopolysaccharide-activated PBNIC but enhanced the proliferation of phytohemagglutinin-activated PBNIC at concentrations of < 1 mg/mL, (2) significantly augmented the expression of CD25 on lymphocytes at concentrations of 0.4 mg/mL or above (p < 0.05), (3) dose dependently (0.1-1.0 mg/mL) activated macrophage phagocytosis and monocyte synthesis of H2O2 and (4) significantly increased the production of cytokines IL-8, IL-10, IL-12 and IL-1 beta at various concentrations of VI-28 (p < 0.05). The results suggest that VI-28 is a potential immunomodulator which probably acts through the activation of lymphocytes and monocytes. Copyright (c) 2006 John Wiley & Sons, Ltd.
著者Lee SKW, Wong CK, Poon PMK, Ip PSP, Che CT, Fung KP, Leung PC, Lam CWK
期刊名稱Phytotherapy Research
出版年份2006
月份10
日期1
卷號20
期次10
出版社JOHN WILEY & SONS LTD
頁次883 - 888
國際標準期刊號0951-418X
電子國際標準期刊號1099-1573
語言英式英語
關鍵詞immunomodulation; lymphocytes; monocytes; traditional Chinese medicine (TCM); VI-28
Web of Science 學科類別Chemistry, Medicinal; CHEMISTRY, MEDICINAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

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