Epidermal growth factor induction of resistance to topoisomerase II toxins in human squamous carcinoma A431 cells
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摘要Alteration of the epidermal growth factor (EGF) signaling pathway occurs frequently in human cancer cells and may subsequently affect the cell survival towards anticancer agents. To elucidate the effect of long-term EGF treatment on the chemo-sensitivity of human cancer cells, human squamous carcinoma A431 cells (AP) were incubated continuously with 50 ng/ml EGF for 30 weeks and these cells were designated as the AC cells. The long-term EGF treatment did not alter the EGFR level and the EGF-induced protein tyrosine phosphorylation pattern in the AC cells. By MTT assay, the AC cells were shown to be more resistant than the AP cells to doxorubicin, etoposide and amsacrine but not to cisplatin. Among the drug-resistant proteins, topoisomerase II alpha (topoII) was downregulated in the AC cells while there was no apparent change in the levels of P-glycoprotein, MRP-1 or glutathione- S-transferase-pi as compared to the AP cells. Furthermore, knockdown of topoII by antisense topoII oligonucleotide transfection decreased the sensitivity to doxorubicin, etoposide and amsacrine in the A431 cells. Results from the present study support an idea that long-term treatment with EGF may induce drug resistance in cells through the downregulation of topoII.
著者Tsang WP, Kong SK, Kwok TT
期刊名稱Oncology Reports
出版年份2006
月份10
日期1
卷號16
期次4
出版社PROFESSOR D A SPANDIDOS
頁次789 - 793
國際標準期刊號1021-335X
電子國際標準期刊號1791-2431
語言英式英語
關鍵詞A431 cells; epidermal growth factor; topoisomerase
Web of Science 學科類別Oncology; ONCOLOGY

上次更新時間 2021-18-04 於 00:42