Cell death induced by MPPa-PDT in prostate carcinoma in vitro and in vivo
Publication in refereed journal

香港中文大學研究人員

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摘要Lack of effective photo sensitizers has become a major limit for extensive application of photodynamic therapy. In this study, the photocytotoxicity and mode of death induced by a newly developed photosensitizer MPPa, a derivative of chlorophyll a, were investigated in PC-3M cell line, a highly metastatic variant of poorly differentiated androgen-independent proctance adenocarcinoma PC-3. MTT reduction assay was used to measure cytotoxicity in both PC-3M and HUVEC, after which a flow cytometer was used to measure apoptotic rate and cell cycle, and then Caspase-3, -8, -9 were investigated. Finally, an animal model was set up to embody the curative effect and for histopathological examinations. The photocytotoxicity of MPPa showed both light- and drug-dose dependent characteristics and no significant dark cytotoxicity was observed in PC-3M cells. In HUVEC, MPPa exhibited an obviously low cytotoxicity. By other in vitro studies, we found MPPa-PDT induced apoptotic mainly via the mitochondrial/Caspase-9/Caspase-3 pathway and could restrain the cell cycle progression from the more sensitive G(0)/G(1)-phases. In vivo, the tumour growth was significantly inhibited after PDT, and many apoptotic cells could be seen by histopathological examinations. These results indicate the death way of cells induced by MPPa is mainly via mild apoptotic and the cure effect is obvious, suggesting that MPPa is a potential photosensitizer of photodynamic therapy for prostate cancer. (c) 2006 Elsevier Inc. All rights reserved.
著者Tian YY, Leung W, Yue KM, Mak N
期刊名稱Biochemical and Biophysical Research Communications
出版年份2006
月份9
日期22
卷號348
期次2
出版社ACADEMIC PRESS INC ELSEVIER SCIENCE
頁次413 - 420
國際標準期刊號0006-291X
電子國際標準期刊號1090-2104
語言英式英語
關鍵詞cell death; photodynamic therapy; prostate carcinoma
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

上次更新時間 2020-30-11 於 01:23