The 3a protein of SARS-coronavirus induces apoptosis in Vero E6 cells
Refereed conference paper presented and published in conference proceedings


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AbstractAn outbreak of severe acute respiratory syndrome (SARS) occurred in China and the first case emerged in mid November 2002. The etiologic agent of this disease was found to be a previously unknown coronavirus, SARS-CoV. The detailed pathology of SARS-CoV infection and the host response to the viral infection are still not known. The 3a gene encodes a non-structural viral protein which is predicted to be a transmembrane protein. In this study, we showed that the 3a protein was localized to the endoplasmic reticulum (ER) in 3a-transfected monkey kidney Vero E6 cells. In vitro experiments of chromatin condensation and DNA fragmentation suggest that the 3a protein may trigger apoptosis. Our data show that over-expression of a single SARS-CoV protein can induce apoptosis in vitro. Thus GFP-3a fusion protein could also be used as a biosensor for monitoring the cytopathic features of SARS infection, e.g. lymphopenia, in animal model systems, similar to nucleocapsid and 7a proteins.
All Author(s) ListWaye MMY, Law PTW, Wong CH, Au TCC, Chuck CP, Kong SK, Chan PKS, To KF, Lo AWI, Chan JYW, Suen YK, Chan HYE, Fung KP, Sung JJY, Lo YMD, Tsui SKW
Name of Conference27th Annual International Conference of the IEEE-Engineering-in-Medicine-and-Biology-Society
Start Date of Conference31/08/2005
End Date of Conference03/09/2005
Place of ConferenceShanghai
Country/Region of ConferenceChina
Journal namePROCEEDINGS OF THE 20TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOL 20, PTS 1-6
Detailed descriptioned. by Scientific Committee of IEEE.\n\nTo ORKTS: http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=1616242&isnumber=33900
Year2005
Month1
Day1
PublisherIEEE
Pages7482 - 7485
ISBN0-7803-8740-6
ISSN1094-687X
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesEngineering; Engineering, Biomedical

Last updated on 2020-02-08 at 03:37