Metallothionein gene expression in zebrafish embryo-larvae and ZFL cell-line exposed to heavy metal ions
Publication in refereed journal

香港中文大學研究人員

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摘要The aim of this study is to investigate the induction of zebrafish metallothionein (zMT) gene expression following the administration of different metal ions using in vivo and in vitro models. The zebrafish embryo-larvae were used for the in vivo study, and NIT gene expression was studied during the development from fertilization (8hpf) to embryo-larval stage using real-time PCR. The LC50 values and zMT mRNA levels were also measured in embryo-larvae exposed to various metal ions. The general trend of 24 h LC50 values as determined is Cu2+ < Hg2+ < Cd2+ << Zn2+. However, Hg2+ was found to be the most potent metal inducer with the highest level of zMT mRNA induction (40-50 folds) in 8hpf embryo-larvae, followed by Cd2+ (similar to 20 folds); Cu2+ and Zn2+ only gave similar to 5 fold of induction. In the in vitro study of ZFL cell-line, Cd2+ is the most potent inducer of zMT mRNA (up to 250 folds), Cu2+ and Zn2+ gave similar potency of similar to 50-100 folds, and Hg2+ gave similar to 40-50 folds of zMT mRNA levels over the control group. (c) 2006 Elsevier Ltd. All rights reserved.
著者Chan KM, Ku LL, Chan PCY, Cheuk WK
會議名稱13th International Symposium on Pollutant Responses in Marine Organisms (PRIMO 13)
會議開始日19.06.2005
會議完結日23.06.2005
會議地點Alessandria
期刊名稱Marine Environmental Research
出版年份2006
月份1
日期1
卷號62
出版社ELSEVIER SCI LTD
頁次S83 - S87
國際標準期刊號0141-1136
語言英式英語
關鍵詞biomarker; mRNA; sub-lethal effects; toxicity
Web of Science 學科類別Environmental Sciences; ENVIRONMENTAL SCIENCES; Environmental Sciences & Ecology; Marine & Freshwater Biology; MARINE & FRESHWATER BIOLOGY; Toxicology; TOXICOLOGY

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